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VOL. 10, ISSUE 4 (2025)
Comparative phytochemical screening of Rhynchostylis retusa (L.) Blume from wild and in vitro origins: An investigation of secondary metabolites
Authors
Somaya Emrog Nayma, Md. Shahin Islam, Momena Khanam Mumu, Minhajur Rahman and Tapash Kumar Bhowmik
Abstract
Rhynchostylis retusa
(L.) Blume is a medicinal epiphytic orchid valued in traditional medicine
across South and Southeast Asia. Owing to habitat destruction, overharvesting
and slow natural regeneration, in vitro culture techniques offer a
promising alternative source for bioactive metabolites. This study
comparatively evaluates the phytochemical composition of naturally grown and in
vitro propagated R. retusa using qualitative screening techniques. Wild
plant parts (leaf, stem, root) and in vitro derived tissues (plantlets,
shoot buds, callus and SPSs) were analyzed for alkaloids, flavonoids,
terpenoids, steroids, quinones, coumarins, tannins, saponins, phlobatannins,
phenols, anthraquinones and cardiac glycosides. Marked differences were
observed between wild and in vitro samples. Roots of wild plant parts
exhibited maximal (+++) accumulation of terpenoids, quinones, steroids,
flavonoids and coumarins, supporting their ethnomedicinal applicability. In
vitro derived tissues accumulated higher levels of tannins and saponins
(+++), suggesting culture induced modulation of the phenylpropanoid pathway.
Glycosides were absent in all samples. The study highlights the phytochemical
richness of R. retusa and confirms that tissue culture can generate
valuable secondary metabolites, positioning in vitro systems as
sustainable sources for pharmaceutical applications.
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Pages:56-58
How to cite this article:
Somaya Emrog Nayma, Md. Shahin Islam, Momena Khanam Mumu, Minhajur Rahman and Tapash Kumar Bhowmik "Comparative phytochemical screening of <i>Rhynchostylis retusa</i> (L.) Blume from wild and <i>in vitro </i>origins: An investigation of secondary metabolites". International Journal of Advanced Research and Development, Vol 10, Issue 4, 2025, Pages 56-58
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