An attempt has been made to study the Physico-chemical assessment of the ground waters of Agra district (U.P.). Eight water samples out of them 4 from hand pumps (HP) and 4 from the tube wells (TW) were collected from different villages around the main city of Agra District. The duration of the study was from January 2022 to June 2022. The physico-chemical parameters analyzed were pH, temperature, turbidity, DO, BOD, COD, chlorides, fluorides, sulphates, nitrates and total hardness. It was observed that all other parameters are within range when compared with WHO standards, only fluorides and chlorides were present in excess amount in tube well water of Rohta village. It has also been observed that tube well water samples have higher fluoride ions concentration as compared to hand pump water samples.