Biosecurity in aquaculture is a novel idea for preventing infectious diseases of aquatic animals. The study inspected the existing biosecurity status and health management practices in 24 commercial farms located in selected upazilas (viz., Mymensingh Sadar, Trishal, and Muktagacha) under Mymensingh district. Data were collected through participatory rural appraisal (PRA) tools such as questionnaire interview, focus group discussion (FGD) and key informant interview. The results revealed that monoculture was similarly preferred as polyculture (50%) by the respondents. However, a good proportion (29.16%) of the farmers did not have decent understanding on biosecurity issues. Biosecurity measures such as sprays, footbath and sanitary facility, showers, disinfection of transport equipment, protective clothing for visitors and quarantine facilities were absent in those farms. However, majority (83.33%) of the farmers had protected dykes and boundary fences in their farms. Bio hygiene measures such as control of rodents and insects in feed storage, water exchange in the pond, control of predators/pests in farm and disinfection of equipment used for culture operation etc were also followed on a very limited basis. Most farmers (87.5%) have stocked diseased free fry from various hatcheries while a few (8.33%) supplied from their own hatchery. Preventive measures such as regular health checking (66.67%), drying of pond (66.67%) and applying lime in the pond (100%) were practiced in the studied farms. About 66.67% farmers used probiotics in their farms for beneficial purposes like water quality management, growth enhancement and disease resistance. Importantly, farmers who at least partially followed biosecurity measures obtained better fish production than those who did not. The findings revealed preliminary information about the general biosecurity and current health management status of Mymensingh farms which demands further information for sustainable fish production.