Seeds of medicinally important orchid Staurochilus ramosum (Lindl.) Seidenf. cultured on four 0.8% (w/v) agar solidified KC; MS; PM and VW basal media. The highest seed germination (86.77%) was achieved on PM medium followed by MS (80.00%); VW (66.67%) and KC (60.00%) media. Time required minimum for seed germination was recorded on PM (10.30±0.24^a weeks) followed by MS (11.20±0.33^b weeks), VW (12.73±0.29^c weeks) and KC (14.30±0.31^d weeks) media. The well-developed rooted seedlings were transferred to outside environment by successive phases of acclimatization and watered regularly. In vitro developed callus, SPSs, shoot buds and root, stem, leaf of naturally grown orchid were used for comparative phytochemical screening. Root and leaf sample of natural Staurochilus ramosum gave the highest precipitation followed by in vitro SPSs, callus or shoot buds, respectively. All in vitro and natural sample gave mild responses (-/+) in Phlobatannin test and responded strong (+++) in Tanins and Terpinoids test. Naturally grown root exhibited best responses in secondary metabolite tests followed by stem and leaf sample.