Objective: The aim was to assess the antimicrobial efficacy of triphala gel at various concentrations against primary plaque colonizers. Material & Methods: Triphala gel was prepared at various concentrations 6.25%, 12.5%, 25%, 50%.The antimicrobial efficacy testing of triphala gel against these bacteria was done by agar well disc diffusion method. Results: All the concentrations inhibited the growth of primary plaque colonizers maximum zone of inhibition was seen at 50% for all the primary plaque colonizers. Conclusion: Triphala gel has the potential to inhibit the growth of primary plaque colonizers and can be used as an antiplaque agent and used in prevention of dental and periodontal diseases.