The study was conducted in 12 clinical cases presented to TVCC, Bidar. Twelve animals were randomly divided in to two groups viz., Group-I and Group-II consisting of six animals in each group. Group –I animals received romifidine (10µg/kg) intravenously, 10minutes later ketamine was given @3mg/kg body weight intravenously for induction of anaesthesia. In Group-II animals xylazine (0.1mg/kg) was administered intravenously, 10 minutes later ketamine was given @3mg/kg body weight intravenously for induction of anaesthesia followed by immediate intubation, maintenance of anaesthesia was done under isoflurane in both the groups. Anaesthetic combinations were compared by clinical and biochemical observations. The induction and recovery were smooth and uneventful in both the groups. Induction was quicker in group-I animals. Clinical and biochemical parameters like induction time, onset of sedation, analgesic score, degree of muscle relaxation, palpebral reflex and recovery time were fluctuated within normal limit. Induction time was quicker and faster followed by onset of sedation was earlier in group-I. Creatinine, alanine transaminase, aspartate transaminase and serum urea nitrogen were fluctuated within normal range in both the groups. In conclusion romifidine as pre-anaesthetic for ketamine and isoflurane anaesthetic maintenance was a better combination than xylazine –ketamine and isoflurane anaesthetic maintenance in cattle.