Protein-specific information of immune cells play an important role in defining new targets and biomarkers for many diseases and an uncontrolled growth of a cell. An initial event that lead to uncontrolled growth during cancerous condition is stimulation of cells by foreign molecules or mitogens and hence it would be a great importance if new proteins markers are identified that are differentially expressed in activated lymphocytes. In this study, we have compared both qualitative and quantitative expression of proteins from normal PBMCs before and after their activation by PHA and Con A lectins. Electrophoresis of proteins from normal resting and lectin-activated PBMCs revealed that two proteins with 95 and 185 kDa are exclusively expressed on PHA-activated PBMCs. Similarly, proteins with Mr 68, 70, and 210 kDa are only part of Con A stimulated PBMCs. On the contrary, protein bands with approx. Mr 35, 125, 170, 200, 250 and 280 kDa were exclusively expressed in resting lymphocytes while disappeared after activation process. Further, quantification of proteins by Image J software reveled that level of protein expression has also changed drastically in activated PBMCs when compared to resting PBMCs. These results provide an insight into the fact that proteins that are expressed or altered during activation process could be the direct target for development of new drugs for blood cancer.