By considering the current regulatory requirement for an analytical method development, a reversed phase high performance liquid chromatographic method for routine analysis of daclatasvir in capsule dosage form has been developed and validated. A simple, precise, rapid and accurate reverse phase HPLC method was developed. A column of Inertsil ODS-3V C18, 250x4.6mm i.d with 5μ particle size was used. The mobile phase comprises of 0.01M Ammonium acetate with pH adjusted to 3.5 (mobile phase solvent-A) and methanol (mobile phase solvent-B) in the ratio of 20: 80 (v/v).The flow rate was 1.0 ml/min and the effluents were monitored at 284 nm. The retention time was 7.79 min. The detector response was linear in the concentration of 100-300µg/ml. The respective linear regression equation being Y= 28817.742X-14741.2. The limit of detection (LOD) and limit of quantification (LOQ) for were found to be 0.05µg/ml and 0.15µg/ml respectively. The assay was found to be 99.85%.The method was validated by determining its accuracy, precision, system suitability, LOD and LOQ. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of daclatasvir in bulk drug and in its pharmaceutical dosage form.