Medical facility is essential to protect the health and well-being of human life. However, the refuse, which produced by medical activities may be toxic, hazardous, or even lethal because of their great risk of diseases transmission. The hazardous and toxic nature of refuse from medical facilities commonly known as “Bio medical waste” consists infectious, radioactive material as well as needles, knives, scalpels etc. constitute a high risk, if these are not properly treated/disposed or allow mixing with other municipal waste. Bio medical waste has grave potential to enhance the growth of pathogen and vectors and it is capable to contaminate other nonhazardous/ non-toxic municipal waste. Hospital waste have high risk of toxic trace metals, toxic chemicals, pathogenic viruses and bacteria (Chintis et al., 2004), The handlers of waste such as rag pickers and waste workers directly or indirectly come into the contact with such hazardous material for economical benefits. Reuse of this hazardous material can be harmful and even fatal due to diseases like cholera, plague, tuberculosis, hepatitis; AIDS (HIV), diphtheria etc. Therefore the main focus of the study is to know the status of awareness among the handlers of biomedical waste.