Foliar anatomical observations are extended for 12 species belonging to 10 genera of the Acanthaceae. Leaves are generally bifacial. The diversity with respect to vascular tissue, occurrence and development of collenchyma, sclerenchyma, palisade and spongy tissues, cell inclusions, etc. are studied in detail. The leaves are although basically similar, they vary species to species and hence appear useful taxonomically significant at specific levels.