This study includes the Preliminary Phytochemical screening and quantitative analysis of secondary metabolites of leaves and stems extract of Mentha arvensis and Azadirachta indica. In this study we were checked the presence of various active metabolites like-alkaloid, tannins, terpenoid, steroid, saponin, anthraquinones, glycosides, flavanoids and phlobatannins. The result of preliminary Phytochemical screening of Mentha arvensis (leaves and stem) showed the presence of various secondary metabolites like alkaloid, tannins, terpenoid, steroid, saponin, anthraquinones, terpenoid, glycosides and flavanoids. Azadirachta indica (leaves and stem) also showed the presence of various secondary metabolites like alkaloid, tannins, terpenoid, steroid, saponin, anthraquinones, saponin, anthraquinones and glycosides. In quantitative analysis the leaves of Mentha arvensis showed the maximum presence of phenolic content (260.995±0.004710645) and flavonoid content was (86.282±0.001).