Prevention and therapy of thrombotic diseases have attracted much attention in developed countries during recentyears. Lysis of preformed fibrin could be accomplished in vivo by plasmin enzyme led to an alternative enzyme-based approach. The drugs used for this therapy are called the fibrinolytic enzymes. In this study, we aimed to screen the ability of Citrobacter sp.(125 sp.) for Fibrinolytic proteaseproduction. Primary and secondary screening process was utilized to select the Citrobacter sp.which give the highest production of fibrinolytic enzyme and was found that Citrobacter braakii (BGCC#2123) had the highest productivity of the enzyme (35 FU/mg) and wasdesignated as CbFP.The maximum activity of the enzyme was observed at temperature 30 ⁰C and pH 8.0. Along with fibrinolytic activity Citrobacter braakii (BGCC#2123) also posses gelatinase activity.Citrobacter braakii (BGCC#2123) may be considered as a new source for thrombolytic agents.