The purpose of this work was to investigate the effect of Majoon-e-Dabeed-ul-Ward (MD) on ethanol (EtOH) induced cytotoxicity in human liver cells. Cells were treated with either EtOH (30mM) alone or together with MD (25μM) for 12 hours. It was found that exposure with EtOH induced different cytotoxicity processes like formation of oxygen radical formation (ROS), decrease in the activities of SOD and Catalase, lactate dehydrogenase leakage, lipid peroxidation, decrease in glutathione (GSH) and increased oxidized glutathione (GSSG) levels in liver cells as compared to untreated cells. EtOH induced lactate dehydrogenase (LDH) leakage was significantly prevented by MD treatment. Activity of Superoxide dismutase (SOD) and catalase were significantly increased in EtOH challenged cells upon treatment with MD. Other beneficial effects associated with MD treatment in EtOH challenged cells were reduction in generation ROS and lipid peroxidation. Our findings suggest that MD exert cytoprotective action against EtOH induced liver cell damage.