Plants diversity in nature is a major segment of biodiversity. It is determined by surrounding of the plants. Every one plant species in nature always trying to maintain their population as well as existence in nature for long time. For such aim these are producing seeds and also adapted to multiply by vegetative modes using root, stem, leaf etc. These modes are variable among the different plant species. The plant Helicteres isora Linn is well adapted to regenerate using its mature stem cutting. Stem cutting 25 - 30 cm length were selected and oblique cut made than grown in the prepared beds till 8 cm depth and in prepared sixty poly bags individually for further development in Herbal garden. Above practice is supporting its better dissemination in the required sites.