The art of dyeing by natural colours has roots in the dawn of civilization. The paper sheds light on 53 plant species from khandesh region of Maharashtra (India) having potential. It seems that the demand for natural dyes is increasing in recent time due to their safe and anti – allergic effects. The information gathered from khandesh region after comparative study and analysis, will throw more light on plant wealth with particular emphasis of dye –yielding plant species. This, in turn, will be useful to the researchers in connection with R & D work of these plant sources.