The immune system modulation by using medicinal plants can provide an alternative to conventional chemotherapy for a variety of diseases. The present study investigates the role of different bioactive compounds in the modulation of immune responses. The study was carried out by using spleenocyte proliferation assays. Macrophage preparations were used for the determination of immunomodulatory potential of pure compounds (Iridin, Irigenin, Tectorigenin). The effect was determined by estimating the concentration of cytokines (IL8, IL10, TNF-α). The nitrite content in the peritoneal macrophages was done by using Griess reagent. Moreover, the enhancement in CD4 and CD8 cell populations was revealed by flow cytometry. The results showed that out of the tested compounds, only Tectorigenin potentially enhanced immune response via augmenting Th1/Th2 response in a dose dependent manner. This study may contribute to the traditional claims of Iris plant species based natural products used for the treatment of various types of infectious diseases.